A single beam could hold a particle in water
Ashkin, Dziedzic, Bjorkholm and Chu’s 1986 author abstract reports single-beam gradient-force trapping of dielectric particles in water. The historical result established stable optical confinement over a broad particle-size range. The 2018 recognition also concerns applications to biological systems.
The field transfers momentum through the particle. For suitable dielectric contrast and focusing, the restoring gradient contribution can overcome destabilizing scattering forces. A bright focus alone does not guarantee stable confinement along every direction.
The bead keeps moving in a stable trap
Near equilibrium a stable trap can often be approximated as a spring. A bead in a viscous fluid then fluctuates under thermal forcing. Those fluctuations contain information about stiffness, but only after detector units have been converted to displacement.
For an overdamped harmonic trap in an equilibrium Newtonian fluid, the corner frequency relates stiffness to drag. The symbols below denote trap stiffness, drag coefficient and frequency in cycles per second. Close surfaces, nonspherical particles or viscoelastic media alter the simple drag model.
Sources: [2]
Two calibrations can disagree for a physical reason
Equipartition uses displacement variance and temperature. Power-spectrum calibration uses the spectral roll-off together with a drag model; an active sinusoidal drive can supply additional information about detector conversion. The cited original experiment compares these approaches rather than declaring them universally interchangeable.
Drift adds low-frequency variance, finite exposure blurs motion, sampling aliases high-frequency fluctuations, and local heating changes temperature. A good-looking Lorentzian fit can therefore coexist with a biased force scale.
Sources: [2]
A force estimate needs a working range
Within a calibrated approximately harmonic range, force is opposite displacement from the trap center. Larger excursions need the actual force-displacement relation and may end in escape. The equilibrium variance formula below assumes a single harmonic coordinate at known temperature.
A molecular force trace further depends on bead linkage, compliance, bandwidth and geometry. Neither an optical-tweezer photograph nor a trapped bead by itself supplies a molecular force measurement.
Sources: [2]
A magneto-optical trap is a different preparation
The existing atom-cooling Lab illustrates radiation forces on atoms with internal transitions and magnetic fields. It has no bead-in-fluid Brownian calibration. Read it as a comparison of optical forces; the independent optical-tweezer measurement remains a separate apparatus and inference task.