Phase gradient and deflection
The sign is for a negatively charged electron moving along positive z.
E47 · Magnetic electron imaging
Why does a magnetic domain wall switch from bright to dark when the defocus is reversed? Because defocus turns the curvature of the electron phase into brightness, and reversing the defocus flips the sign of that conversion. At exact focus a pure-phase film shows no contrast at all. Electric phase, for example from changes in thickness, can produce similar Fresnel contrast, so a line that flips is not by itself proof of a magnetic wall.
Defocus the image of a magnetic foil, flip the sign of the defocus, and use a Foucault aperture to pass only half of the deflection angles. Compare the counted images with the known induction, then check whether an electric phase can mimic a magnetic signal.
Physics tutorial
BackgroundA thin magnetic foil barely changes the electron intensity. It mainly shifts the phase of the wave and deflects the electrons slightly. Lorentz TEM makes the domains and their walls visible by defocusing the image or by blocking part of the deflected beam.
Why it mattersThe goal: use positive Fresnel defocus with the electric potential at zero. Record at least 1000 counts per pixel on average, reach a model RMS contrast of at least 0.12, and get an opposite-defocus contrast correlation of -0.85 or lower.
Start with the essentials
The sign is for a negatively charged electron moving along positive z.
Before the aperture, propagation keeps the total expected intensity unchanged.
This approximation explains why the contrast reverses with the sign of defocus; the image itself is computed with full complex-wave propagation.
Typical misconceptionThe known reference map is the field the microscope recorded or recovered.
Better mental modelThe reference map is the prescribed specimen input. The images are counted and processed without it; the reference is used only to score the error.
Start from the In focus preset, then set the Signed defocus first positive, then negative.
What to observe: Defocus turns the phase into visible contrast. Flipping the sign roughly swaps bright and dark, but at finite defocus the swap is only approximate.Set Image mode to Foucault, flip the Accepted angular sign and raise the Foucault angular cutoff.
What to observe: The domain contrast changes, and fewer electrons reach the detector.Load the Electric phase confound preset, move the Applied field slider, then reach the target.
What to observe: A wall-like line of intensity is not enough, by itself, to show that magnetism caused it.